If a plugin loads when a webpage load then only the load-time speed will be effected. For example, Form 7 plugin loads with the load of contact form load in a webpage. In your case, plugin is not loading with the webpage. So, there won't be any impact on speed of the webpage loading whether that plugin gets activated or not.

Though a WordPress developer might have a better idea to speed up the website.

I Optimized Html & CSS code and images sizes in wordpress with the help of WP-Optimize plugin so because of the plugin is active the http request is taking lot of time. my question is if i deactived this wp-optimize plugin it will change the effect on the performance of the website

Once again, welcome to SEO Forum!

It's a pleasure for me to welcome you here, in our own tiny piece of the internet where we meet to brainstorm and exchange our experience with nearly anything, but mostly connected with internet marketing, search engine optimization and related topics.

To make sure you enjoy your stay, this is thread for you - guys with ideas. Feel free to suggest new features, changes and edits to the site, so we can grow together and make this even better and nicer place to be.

I will personally try to go through all of your requests, and prioritize them according to how you upvote.

Don worry to post anything. Really.

Thank you, @seoradar for your beautiful suggest.